Sigma Aldrich A3553 Acrylamide suitable for electrophoresis, ≥99% (HPLC), powder Cas 100 gr
Acrylamide
for electrophoresis, ≥99% (HPLC), powder
Synonym: 2-Propenamide, Acrylic acid amide
-
CAS Number 79-06-1
-
Linear Formula CH2=CHCONH2
-
Molecular Weight 71.08
-
Beilstein/REAXYS Number 605349
-
EC Number 201-173-7
-
MDL number MFCD00008032
-
eCl@ss 39031205
-
PubChem Substance ID 24890783
-
NACRES NB.22
Properties
| Related Categories | Acrylamides, Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Protein Electrophoresis, Life Science Reagents for Protein Expression and Purification, |
| grade | for electrophoresis |
| vapor density | 2.45 (vs air) |
| vapor pressure | 0.03 mmHg ( 40 °C) |
| InChI Key | HRPVXLWXLXDGHG-UHFFFAOYSA
|
| assay | ≥99% (HPLC) |
| form | powder |
| impurities | ≤0.005% Acrylic acid |
| ≤0.005% Insoluble in water | |
| conductivity | ≤10 μmho (35% solution) |
| bp | 125 °C/25 mmHg (lit.) |
| mp | 82-86 °C (lit.) |
| solubility | water: soluble 2.5 g/10 mL, clear, colorless |
| cation traces | Fe: ≤1 ppm |
| Mg: ≤3 ppm | |
| heavy metals (as Pb): ≤5 ppm |
Description
General description
Acrylamide functions as a quencher of tryptophan fluorescence.[1] Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels,[3] which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers.[3] Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.[1]
Application
Acrylamide has been used in the preparation of polyacrylamide gels.[2][4]
Packaging
1 kg in glass bottle
100, 500 g in glass bottle
Compatibility
This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.
Safety Information
