Sigma Aldrich A3553 Acrylamide suitable for electrophoresis, ≥99% (HPLC), powder Cas 100 gr
Acrylamide
for electrophoresis, ≥99% (HPLC), powder
Synonym: 2-Propenamide, Acrylic acid amide
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CAS Number 79-06-1
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Linear Formula CH2=CHCONH2
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Molecular Weight 71.08
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Beilstein/REAXYS Number 605349
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EC Number 201-173-7
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MDL number MFCD00008032
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eCl@ss 39031205
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PubChem Substance ID 24890783
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NACRES NB.22
Properties
| Related Categories | Acrylamides, Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Protein Electrophoresis, Life Science Reagents for Protein Expression and Purification, |
| grade | for electrophoresis |
| vapor density | 2.45 (vs air) |
| vapor pressure | 0.03 mmHg ( 40 °C) |
| InChI Key | HRPVXLWXLXDGHG-UHFFFAOYSA
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| assay | ≥99% (HPLC) |
| form | powder |
| impurities | ≤0.005% Acrylic acid |
| ≤0.005% Insoluble in water | |
| conductivity | ≤10 μmho (35% solution) |
| bp | 125 °C/25 mmHg (lit.) |
| mp | 82-86 °C (lit.) |
| solubility | water: soluble 2.5 g/10 mL, clear, colorless |
| cation traces | Fe: ≤1 ppm |
| Mg: ≤3 ppm | |
| heavy metals (as Pb): ≤5 ppm |
Description
General description
Acrylamide functions as a quencher of tryptophan fluorescence.[1] Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels,[3] which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers.[3] Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.[1]
Application
Acrylamide has been used in the preparation of polyacrylamide gels.[2][4]
Packaging
1 kg in glass bottle
100, 500 g in glass bottle
Compatibility
This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.
