Description
Catalogue Number	109218
Description	GRAM'S CRYSTAL VIOLET SOLUTION FOR THE G
Overview	The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange. A bottle with 500 ml Gram's crystal violet solution is sufficient for staining up to 250 slides. Gram's crystal violet solution is also available as part of the Gram-color stain set. It is an IVD product and CE registered.

Product Information
HS Code	3204 19 00

Physicochemical Information
Density	0.99 g/cm3 (20 °C)
Flash point	47 °C

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H226: Flammable liquid and vapour. H412: Harmful to aquatic life with long lasting effects.
Precautionary Statement(s)	P210: Keep away from heat. P273: Avoid release to the environment.
Storage class	3 Flammable liquids
WGK	WGK 1 slightly water endangering
Disposal	28 Aqueous solutions: Container D.

Safety Information
Categories of danger	flammable, dangerous for the environment
R Phrase	R 10-52/53 Flammable.Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
S Phrase	S 61 Avoid release to the environment. Refer to special instructions/ Safety data sheets.

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Transport Information
Declaration (railroad and road) ADR, RID	UN 1993 , 3, III
Declaration (transport by air) IATA-DGR	UN 1993 , 3, III
Declaration (transport by sea) IMDG-Code	UN 1993 , 3, III

Specifications
Suitability for microscopy	passes test
Staphylococcus aureus	dark blue-violet
E.coli	pink to red

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MERCK 109218 Gram's crystal violet solution for the Gram staining method

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