Fraser Listeria Selective Supplement Merck 10009310 X 1 Vial

Fraser Listeria Selective Supplement Merck 10009310 X 1 Vial
Catalogue Number	100093
Replaces	1.00093.0017

Product Information
HS Code	3821 00 00
Quality Level	MQ100

Physicochemical Information
pH value	8.1 - 8.6 (H₂O, 20 °C) Aqueous solution

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Safety Information
Hazard Symbols	Harmful
Categories of danger	irritant, dangerous for the environment, toxic for reproduction
R Phrase	R 41-52/53-63 Risk of serious damage to eyes.Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.Possible risk of harm to the unborn child.
S Phrase	S 26-36/37/39-61 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.Wear suitable protective clothing, gloves and eye/face protection.Avoid release to the environment. Refer to special instructions/ Safety data sheets.

Storage and Shipping Information
Storage	Store at +2 °C to +8 °C.

Transport Information
Declaration (railroad and road) ADR, RID	Kein Gefahrgut
Declaration (transport by air) IATA-DGR	No Dangerous Good
Declaration (transport by sea) IMDG-Code	No Dangerous Good

Specifications
Growth promotion test according to ISO 11133 Mixed inoculum of Listeria, E.coli and E.faecalis Additionaly monoculture of E.coli and E.faecalis Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium - Listeria monocytogenes ATCC 19111 (WDCM 00020)	10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013)	≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012)	≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	≥ 1000
Blacking - Listeria monocytogenes ATCC 19111 (WDCM 00020)	+
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	+
- Escherichia coli ATCC 25922 (WDCM 00013)	-
- Escherichia coli ATCC 8739 (WDCM 00012)	-
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	-
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	-
Growth on ALOA® medium - Listeria monocytogenes ATCC 19111 (WDCM 00020)	> 10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	> 10 CFU
Colonies - Listeria monocytogenes ATCC 19111 (WDCM 00020)	bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	bluegreen with non transparent zone
Growth on Tryptic soy agar - Escherichia coli ATCC 25922 (WDCM 00013)	no growth
- Escherichia coli ATCC 8739 (WDCM 00012)	no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	< 100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	< 100 CFU
Incubation: Half Fraser Broth: 24 hours at 30°C, aerobic Fraser Broth: 48 hours at 37°C, aerobic Confirmation on ALOA®-Medium: 48 hours at 37°C, aerobic Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic