Bovine Serum Albumin
heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)
Synonym: Albumin bovine serum, BSA, Bovine albumin
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CAS Number 9048-46-8
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EC Number 232-936-2
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MDL number MFCD00130384
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NACRES NA.27
Properties
| Related Categories | Albumin, Biochemicals and Reagents, Bovine Serum Albumins, Heat Shock Fractionated, Proteins and Derivatives More... |
| Quality Level | 200 |
| biological source | bovine |
| assay | ≥96% (agarose gel electrophoresis) |
| form | lyophilized powder |
| mol wt | ~66 kDa |
| purified by | heat shock fractionation |
| packaging | poly bottle of |
| origin | USA origin |
| loss | ≤5% |
| pH | 7 |
| solubility | water: soluble (40 mg/ml) |
| storage temp. | 2-8°C |
Description
Packaging
10, 50, 100, 500 g in poly bottle
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Suitability
Suitable for growth of Borrelia burgdorferi in BSK II medium
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.